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  • Image Analysis

    Image-Pro Platform

    • Image-Pro
    • Base Features
    • 2D Capture
    • 2D Measurements
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    • 3D Visualization Analysis
    • AutoQuant Deconvolution

    Image Analysis Solution

    • MA-Pro
      • Grain Size
      • Sphericity
      • Cast Iron
    • IMC Coverage
    • Cleanliness
    • CCL Resin Flow
    • Fiber Cross-Section

    Product Integration

    • Wafer Mapping & Inspection
    • Image Scanning System
    • Stage Conotrol System
    • Extend Depth Of Field
    • Defect Inspection
    • Image Measurement
    • Hardness Testing

    Packages for Image Analysis

    Image-Pro® AI
    Life Science

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    Materials Science

     

    2D Measure Module
    AI Deep Learning

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    Analysis Protocols

    Helpful

    • System Requirements
    • Supported File Formats
    • Version Comparison
  • Optoelectronics

    TOPCON Products

    • 2D Spectroradiometer (SR-5100 Series)
      • SR-5100 2D Spectroradiometer
      • SR-5000 2D Spectroradiometer
    • Spectroradiometer (SR Series)
      • SR-5A Spectroradiometer
      • SR-5AS Spectroradiometer
      • SR-5S Spectroradiometer
      • SR-5 Spectroradiometer
      • SR-UL2 Spectroradiometer
      • SR-NIR Near Infrared Spectroradiometer
    • Luminance Colorimeter (BM Series)
      • BM-7AC Luminance Colorimeter
      • BM-5AC Luminance Colorimeter
      • BM-9A Luminance Meter
    • 2D Luminance Colorimeter (UA Series)
      • UA-200A 2D Luminance Colorimeter
      • UA-10A Uniformity Analyzer
      • UA-20C Luminance & Chromaticity Uniformity Analyzer
      • UA-20Y Luminance Uniformity Analyzer
    • Luminance Colorimeter (RD Series)
      • RD-80SA Luminance Colorimeter
    • Illuminance Meter (IM Series)
      • IM-2D Illuminance Meter
      • IM-600/IM-600M Digital Illuminance Meter
    • Ultraviolet Meter (UVR Series)
      • UVR-T2 Industrial UV Checker
      • UVR-300 UV Radiometer
    • Topcon Calibration Service

    APACER Products

    • Spectroradiometer (Hand-held Type)
      • AL100 / AL110
      • AL250
    • Spectral Irradiance Meter (Hand-held Type)
      • Ai111
      • Ai30S
      • Ai101
      • Ai250
    • Automation Platform for Sprctorradiometer
  • Automation

    Prior Microscope Automation

    • Motorized Stages For Upright Microscopes
      • ProScan H101A Motorized Stages
      • ProScan H101F Motorized Stages
      • ProScan H105 Motorized 6" Travel Stage
      • ProScan H105F Motorized Flat Top 6" Travel Stage
      • ProScan H116 Motorized 8" Travel Stage
      • ProScan H116SPN Motorized 8" Travel Stage with Shuttle Chuck
      • ProScan HWL6AL12 Motorized 8" Travel Stage with Shuttle Chuck
      • ProScan H112 Motorized 12" Travel Stage
      • ProScan H138A Motorized Stage With 8 Slide Capacity
      • ProScan HT1111LC Hardness Testing Motorized Industrial Stage
      • OptiScan ES111 Motorized Stages
    • Motorized Stages For Inverted Microscopes
      • ProScan H117 Motorized Stage
      • ProScan HLD117 Linear Motor Ultra-precision Stage
      • OptiScan ES107 Economical Motorized Stage
    • Physiology Platforms
      •    ── XYZ Deck Physiology Platforms ──   
      • H189 High Precision XYZ Deck
      • HZ106KT1 High Precision XYZ Deck
      • HZ106KT1E High Precision XYZ Deck
      • ProScan HT11 Heavy Duty Motorized Stage
      • ZDP50 Microscope Translation Stage
      •    ── Z Deck Electrophysiology Platform ──   
      • Manual Z Deck Manual XY Stage
      • Motorized Z Deck XY Stepper Motor Stage
      • Encoded Motorized Z Deck
    • Robotic Sample Loaders
      • SL160 Robotic Slide Loader for Microscopy
      • PLW20 Robotic Well Plate Loader System
    • OpenStand® platform
      • OpenStand-V Inverted Microscopy Platform
      • OpenStand-L Large Format Microscopy Platform
      • OpenStand-U Upright Microscopy Platform
      • OpenStand-M Small Format Microscopy Platform

    Prior Imaging Components

    • Focus Mechanisms
      •    ── Piezo-driven Nanoscan ──    
      • NanoScan OP400 Piezo Objective Scanner
      • NanoScan OP800 Piezo Objective Scanner
      • NanoScan SP Piezo Z-Axis Stage
      • WP120A Single Axis Piezo Stage (12" Wafer)
      •     ── Focus Mechanisms ──    
      • FB201 Manual Focusing Block - 29mm
      • FB203 Motorized Focusing Mount - 38mm
      • FB204 / FB205 Stage Focusing Mount - 38mm
      • FB206 Stage Focusing Mount - 38mm
      • FB210 Motorized Focusing Mount - 50mm
      • FB212 Stage Focusing Mount - 50mm
      • PS3H122R Bolt-On Motorized Focus Drive
      • H275LMT Extended Travel Motorized Focus Tower
    • Laser Autofocus
      • PureFocus™850 Laser Autofocus System
    • Controllers & Joysticks
      • ProScan III Universal Multi-axis Controller
      • OptiScan III Affordable 3-Axis Controller
      • ES10ZE Single Axis Focus Controller
      • CS200 Series Joysticks
    • Filter Wheels, Turrets & Shutters
      • HF108 High-Speed 8 Position Filter Wheel
      • HF110 High-Speed 10 Position Filter Wheel
      • HF108IX3 8 Position Filter Wheel for IX3
      • HF6NTK 6 Position Motorized Turret Upgrade Kit for Nikon Ti2
      • HF202HT 25mm High Speed Shutters
    • Light Sources
      • L200 Fluorescence Microscope Illumination

    Queensgate Nanopositioning Devices

    • Nanopositioning Piezo Stages
      • NPS-X-15A/NPS-X-15B Low Profile Fast 20µm Stage
      • NPS-X-28C Titanium Ultra Fast 28µm Stage
      • NPS-Z-15A Ultra Low Drift 15µm Stage
      • SP Mini-500 Compact Sample Positioner
      • NPS-XY-100A
      • NPS-XY-100D
      • NPS-TG-7A Fast Tip Tilt
      • NanoScan OP400
      • NanoScan OP800
      • NanoScan SP Z Series
    • Precision Actuators & Translators
      • DPT-E Closed Loop Piezo Actuators
      • MTP Open Loop Piezo Actuators
      • NPS-Z-15L Ultra High Load Closed Loop 20μm Actuator
      • NPS-Z-500B Long Range 500μm Amplified Actuator
    • Nanosensors
      • NX Series Capacitive Sensors
      • NC Series Capacitive Sensors
    • Nanopositioning Control Electronics
      • NPC-D-5200 Digital Piezo Closed Loop Controller
      • NPC-D-6000 Series Digital Piezo Closed Loop Controller
    • Vacuum Compatible Products
      • DPT-E Closed Loop Piezo Actuators
      • NX Series Capacitive Sensors
      • NC Series Capacitive Sensors
      • NPS-XY-100A
      • NPS-XY-100D
      • NPS-Z-500B Long Range 500μm Amplified Actuator
      • WP120A Wafer Positioner / Scanner
    • Specialist Stages and OEM Solutions
      • NPS-XYP-250Q
      • NPS-Z-90Q
      • NPS2100-20/NPS2101
  • Microscopic

    Microscope Camera

    • Retiga CCD Family
      • MicroPublisher 6™ CCD Camera
      • Retiga R6 CCD camera
      • Retiga ELECTRO Cooled CCD camera
      • Retiga LUMO CCD camera
    • INFINITY Series Microscopy Camera
      • INFINITY 5 CMOS Microscopy Camera
      • INFINITY 8 CMOS Microscopy Camera
    • Photometrics Scientific Cameras
      • Kinetix sCMOS cameras
      • Prime 95B™ Back-illuminated sCMOS camera
      • Prime BSI™ Back-illuminated sCMOS camera
      • Prime BSI Express sCMOS camera
      • Iris Family sCMOS cameras
      • Retiga E Family Cooled & Low-Noise CMOS camera
      • Moment High Speed CMOS camera
    • MI-Cam CMOS Camera
      • MI-Cam 5 Pro 顯微鏡數位相機
      • MI-Cam 6 顯微鏡數位相機
      • MI-Cam 20 顯微鏡數位相機

    Optical Microscope

    • Evident Microscope
    • Nikon Microscope
    • Microscope Cleaning and Maintenance

    Micrometer/Reticles/Scales

    • Microscope Eyepiece Reticles
    • Stage Micrometers & Calibration
    • TEM Grids (Specimen Support Grids)
    • Precision Apertures
    • Optical Resolution Charts
    • Counting Chambers
    • Inspection Instruments & Magnified Scales
    • Film Cover Slips and Film Reticles
  • Contact
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  2. Z-Stack景深合成影像拍照系統

Z-Stack 影像堆疊拍照控制系統

Image Acquire & Extend Depth Of Field for Z Stack System

Z-Stack 影像堆疊拍照控制系統

Image Acquire & Extend Depth Of Field for Z Stack System

Z Stack 系統示意圖

產品應用簡介

 

影像分析的主要問題-影像失焦

影像分析最容易受影像品質的影響,特別是對焦不佳的影像,會讓影像邊緣模糊、色彩混濁、細節分辨不易,

往往會大幅度的影響影像分析的結果,此時即便是再好的影像分析系統,也無法針對正確的目標進行分析,導致分析的結果缺乏準確性與再現性。

影像失焦示例

完美避免影像對焦不佳現象

影像對焦不佳可以很容易的用目測判定,對焦清楚的影像必須是邊緣銳利且可分辨影像細節。

目前多數的顯微拍照設備,都是採用所見即所得的影像拍照方式,因此利用動態的即時預覽畫面,

在取像前小心的調整好對焦狀態,即可拍到不錯的影像。

 

但是有些情況影像失焦是無法避免的,如進行高倍率顯微影像拍照時,因為高倍率物鏡景深較短的關係,

對焦不佳的問題便會發生,特別是遇到檢測樣品表面不夠平坦或樣品表面高低起伏、景深過大時,特別容易有此問題,

這時無論如何調整對焦狀態,都無法清楚的拍到整張對焦清晰的影像。如果細心觀察整張影像的對焦狀態,便會發現影像內某些區域對焦清楚時,某些區域便會失焦模糊。

影像失焦示例
 

 

Z Stack 影像堆疊拍照控制系統

中惠科技 Z Stack 影像堆疊拍照控制系統,整合 Prior 電動控制 Z 軸系統與 Media Cybernetics Image Pro Plus 影像分析軟體,

針對顯微拍照對焦不佳的問題,提供功能強大且自動化的解決方案。利用 Z Stack 影像堆疊拍照控制系統,操作者可以簡易地設定 Z Stack 的拍照區間,

並可設定區隔張數,系統會針對使用者的設定,

自動完成 Z Stack 影像堆疊拍照,並且自動完成最佳對焦影像判定或影像對焦合成 (Extended Depth of Field) 等工作,大幅度地減低人為操作的誤差與不便。

Z Stack 系統示意圖

 

系統組成

Media Cybernetics Image Pro Plus (Z Stack Control Plug-in Module)

Prior 電動控制 Z 軸系統 (參考 Prior 電動 Z 軸)

顯微鏡專用數位相機 (參考顯微拍照設備,如 Optronics 數位相機、QImaging 數位相機等)

研究型金相顯微鏡 (參考 Olympus 研究型金相顯微鏡)

Z Stack 硬體示意圖

 

功能特點

自動化拍照控制與 Z 軸對焦控制、提供 TimeLapse 間隔時間拍照功能

提供 Z Stack 堆疊影像拍照功能、整合 TimeLapse 與 Z Stack 拍照功能

Z Stack 自動完成對焦最佳影像判定、Z Stack 自動完成全景深對焦合成

提供 Z Stack Align 影像對位修正功能、拍照影像可設定自動存檔、可設定 Z 軸對焦範圍與拍照張數

 
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